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D7808 Sigma

PCR Low Ladder Set

marker set for electrophoresis of PCR fragments

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
grade   for molecular biology
form   liquid
usage   50 uses
suitability   suitable for electrophoresis (DNA)
storage temp.   2-8°C

Description

Components

Sigma’s PCR Low Ladder Set is provided as three separate tubes:
1 vial (25 ug) PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial (25 ug) PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial 6x loading buffer (250 ul volume) containing 15% Ficoll, 0.06% bromophenol blue, 0.06% xylene cyanol FF, and 30 mM EDTA.
1 additional empty tube is provided for storing the mixture.

General description

Sigma’s PCR PCR Low Ladder Set contains one vial each of the PCR 100 bp Low Marker and the PCR 20 bp Low Marker. When mixed together, the ladder appears with bands every 20 bp, with every 5th band more intense. About two ul of the mixed ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.5%.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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