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D8045 Sigma

AccuTaq LA DNA Polymerase

High fidelity Taq enzyme, with 10X buffer & DMSO

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Properties

Related Categories Long & Accurate PCR, Molecular Biology, PCR/Amplification More...
form   liquid
feature   hotstart: no
concentration   5 units/μL
color   colorless
  colorless
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Increased fidelity, up to 6.5× that of Taq DNA polymerase
• Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA.

Other Notes

AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.

Store AccuTaq LA DNA Polymerase and the 10x Buffer at –20 °C. Store DMSO at room temperature. Melting frozen DMSO at ~30 °C will not affect performance.

Packaging

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C.

Application

AccuTaq LA DNA Polymerase is utilized to amplify DNA sequences including genomic targets larger than 20 kb, as a result of a mixture of high quality Taq polymerase with a proofreading polymerase.

Produce long DNA amplicons for:
• Mutation analysis
• Cloning genes
• DNA sequencing
• cDNA library generation

Price and Availability


KiCqStart Probe Assays

Kapa Biosystems
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References

Long and Accurate PCR Amplification of DNA D8045 Protocol

Effective denaturation is accomplished by the use of higher temperatures for shorter periods of time or by the use of co-solvents, such as dimethyl sulfoxide. Addition of DMSO in the reaction at a fi...

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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