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D8156 Sigma

Monoclonal Anti-Digoxin antibody produced in mouse

clone DI-22, ascites fluid

Synonym: Monoclonal Anti-Digoxin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Digoxin, Antibodies to Pharmacologicals,
biological source   mouse
antibody form   ascites fluid
clone   DI-22, monoclonal
contains   15 mM sodium azide
application(s)   dot blot: suitable
  flow cytometry: suitable
  indirect ELISA: 1:10,000 using digoxin-BSA
  indirect ELISA: 1:2,500 using digoxigenin-transferrin
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

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Immunogen

Digoxin-KLH.

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Application

Monoclonal Anti-Digoxin antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules.

Biochem/physiol Actions

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.

General description

The antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin. The antibody may be used in various immunochemical techniques including ELISA, dot blot, flow cytometry, DNA hybridization, and in-situ hybridization (ISH).

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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