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D8168 Sigma

Monoclonal Anti-Dystrophin antibody produced in mouse

clone MANDYS8, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   rat, chicken, mouse, pig, human, rabbit
application(s)   indirect ELISA: suitable
  indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
  microarray: suitable
  western blot: suitable
clone   MANDYS8, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human dystrophin fragment.

General description

Dystrophin is a 427kD cytoskeletal protein found in muscle sarcolemma as a membrane-spanning protein complex which connects the cytoskeleton to the basal lamina. Defects in the gene coding for dystrophin leads to X-linked Duchenne muscular dystrophy. Monoclonal anti-dystrophin antibody can be used to study the structure and function of dystrophin and its interactions with other proteins. The antibody can also be used in immunohistochemical analysis for prenatal or post-abortion diagnosis of muscle dystrophies carriers. Mouse monoclonal anti-dystrophin antibody reacts specifically with an epitope present on the rod domain of the human dystrophin molecule.

Antigen Background

The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry-paraffin (1 paper)

Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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