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D8187 Sigma

JumpStart REDTaq® DNA Polymerase

Hot-start Taq enzyme with inert dye, 10X buffer included

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Properties

Related Categories Core Bioreagents, Hot Start PCR, Life Science Reagents for PCR, Molecular Biology, PCR Enzymes,
form   liquid
feature   hotstart
concentration   1 unit/μL
color   red
  red
shipped in   wet ice
storage temp.   −20°C

Description

Application

• Multiplex PCR
• Amplification with reduced non-specific amplification
• High-Throughput PCR

Features and Benefits

• Prevents amplification of nonspecific products, resulting in increased efficiency and higher yields of the desired sequence
• No lengthy activation step is required for enzyme activity to be restored
• Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
• Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye additions

General description

JumpStart REDTaq DNA Polymerase is Sigma’s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.

• The hot start Taq antibody prevents non-specific product formation.
• Assembled PCR reactions can be placed at room temperature up to 2 hours.
• Red tracer allows visible tracking of enzyme addition and reaction mixing.
• PCR can be directly loaded onto an agarose gel without addition of loading buffers.
• The red tracer is a tracking dye that run at 125bp on a 1% agarose gel.

Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.

Other Notes

View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.

Packaging

The enzyme is provided with an optimized 10× reaction buffer.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Price and Availability


KiCqStart Probe Assays

Kapa Biosystems
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction & Historical Timelines - PCR Technologies Guide

The Polymerase Chain Reaction (PCR) is used in all areas of biological science research, including the clinical, forensic and diagnostic fields and the widespread adoption of the PCR technique has re...
Keywords: Amplification, Clinical, Degradations, Detection methods, Diagnostic, Diseases, Forensic, Gene expression, Genetic, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Separation, Transcription

Protocols

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase

Note: The use of DMSO or formamide with JumpStart REDTaq DNA Polymerase is not recommended due to interference with the enzyme-antibody complex. Other co-solvents, solutes (e.g., salts) and extremes ...
Keywords: Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Antibody-Enzyme Mediated Hot Start PCR Protocol

During PCR assay preparation, nonspecific amplification can occur due to binding of PCR primers to nonspecific templates and from formation of primer dimers which result from using other primer molec...
Keywords: AGE, Amplification, Buffers, Electrophoresis, Enzyme activity, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

Applications with REDTaq

Since its introduction, REDTaq has been well received by the scientific community. The original product idea was to add convenience to PCR by formulating a reaction that also contained loading buffer...
by Brian Ward and Keming Song
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Applications, Error, Melting, Methods, Nucleic acid annealing, Peptide synthesis, Polymerase chain reaction, Purification, Sequencing, transformation

Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via:
Keywords: AGE, Amplification, Electrophoresis, Gel electrophoresis, Gene expression, Nucleic acid annealing, Polymerase chain reaction, Size-exclusion chromatography

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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