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D8281 Sigma

Anti-Desmin antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells,
species reactivity   mouse, human, chicken, bovine
application(s)   indirect immunofluorescence: 1:20 using cultured cells
  indirect immunofluorescence: 1:20 using human skeletal muscle sections
  western blot: 1:100 using animal or human skeletal muscle.
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DES(1674)
mouse ... Des(13346)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

desmin from chicken gizzard

General description

Desmin is an intermediate filament protein found mainly in skeletal, cardiac, and smooth muscle cells. It acts as a marker for tumor origin. It also facilitates mechanical support for muscles, mitochondrial localization, myofibrillogenesis, gene expression regulation, and intracellular signalling. Anti-desmin antibody can be used for immunocytochemical localization of intermediate filaments in all types of muscle cells. It can also be used to stain a wide desmin band of 50,000 to 55,000 molecular weight using immunoblotting assays. Rabbit anti-desmin antibody reacts specifically with desmin in bovine, mouse, chicken and human.

Application

Anti-desmin antibody can be used in immunocytochemistry and western immunoblotting. It can also be used in indirect immunofluorescence.

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Safety Information

WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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