|Related Categories||Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Genomic DNA Amplification, Molecular Biology,|
|shipped in||wet ice|
Includes 10× PCR Reaction Buffer
For routine PCR amplification from genomic DNA
• Enhanced amplification on genomic and difficult DNA templates
• Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
• Quick recognition and confirmation of appropriate mixing
• No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.
REDTaq Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The dye migrates slightly faster than bromophenol blue at approximately the same rate as a 125 base pair fragment.
The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
Customers Also Viewed
Hot-start Taq enzyme with inert dye, 10X buffer included
Taq for routine PCR with inert dye, 10X buffer included
Complete PCR reagent with standard Taq DNA Polymerase and inert dye
Certificate of Analysis
Certificate of Origin
REDTaq DNA Polymerase is a convenient package that includes all the necessary components for a PCR reaction except primers, DNA template and water. The formulation has been optimized for amplificatio...
Keywords: AGE, Amplification, Buffers, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Polymerase chain reaction, Purification, Sequencing, transformation
Since its introduction, REDTaq has been well received by the scientific community. The original product idea was to add convenience to PCR by formulating a reaction that also contained loading buffer...
by Brian Ward and Keming Song
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Error, Melting, Methods, Nucleic acid annealing, Peptide synthesis, Polymerase chain reaction, Purification, Sequencing, transformation
Innis, M.A., et al. PCR Protocols: A Guide to Methods and Applications, (1990)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?