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  • D8406 - Monoclonal Anti-Dinitrophenyl antibody produced in mouse

D8406 Sigma

Monoclonal Anti-Dinitrophenyl antibody produced in mouse

IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Dinitrophenyl, Monoclonal Anti-DNP



Related Categories Alphabetical Index, Antibodies, Antibodies to Dinitrophenyl Group (DNP), DE-DL, Molecular Biology,
biological source   mouse
antibody form   affinity purified immunoglobulin
clone   SPE-7, monoclonal
form   buffered aqueous solution
concentration   ~1 mg/mL
application(s)   indirect ELISA: .05-.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.
isotype   IgE
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C


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DNP-KLH conjugate

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Dinitrophenyl antibody is suitable for use in flow cytometry and immunocytochemistry.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

General description

Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Hence, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP antibody is specific for DNP.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

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Peer-Reviewed Papers


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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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