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D8418 Sigma

Dimethyl sulfoxide

for molecular biology

Synonym: DMSO

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Properties

Related Categories β-Glucuronidase Drug Analysis Bundle, Analytical and Industrial Enzymes, Biochemicals, Biochemicals and Reagents, Core Bioreagents,
grade   for molecular biology
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
InChI Key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N
product line   BioReagent
assay   ≥99.9%
form   liquid
autoignition temp.   573 °F
shelf life   Recommended retest period - 2 years
expl. lim.   42 %, 63 °F
impurities   ≤0.001 meq/g Titratable acid
  ≤0.1% water (Karl Fischer)
color   colorless
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
solubility   H2O: miscible (completely)
density   1.10 g/mL(lit.)
absorption   UV absorption/ passes test
suitability   suitable for molecular biology
foreign activity   DNase and RNase, none detected
storage temp.   room temp

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Packaging

1 L in glass bottle

50, 100, 250, 500 mL in glass bottle

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.

Dimethyl sulfoxide has been used-
as an oligonucleotide solvent in array spotting
as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

General description

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
1
RTECS 
PV6210000
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/shippingandlong-termstoragev3.Par.0001.File.tmp/shippingandlong-termstoragev3.pdf
What is the molarity of DMSO?
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
What is the DMSO dissolved in?
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
Where does DMSO come from?
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
Does DMSO have any synthetic utility?
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes. To view this information in the D2650 product information sheet, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d2650pis.Par.0001.File.tmp/d2650pis.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
Is Dimethyl sulfoxide a sterile product?
No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What can I use to solubilize Product D8418, Dimethyl sulfoxide?
This product is miscible in water (1 mL DMSO + 1 mL H2O), yielding a clear, colorless solution. DMSO is a very hygroscopic liquid and should protected from exposure to moisture. DMSO is also miscible with ethanol, acetone, ether, benzene, and chloroform.
Is Product D8418, Dimethyl sulfoxide, stable under extreme conditions?
DMSO is stable up to 100°C in alkaline, acidic and neutral conditions. At temperatures approaching its boiling point, DMSO is stable in neutral or alkaline conditions.
Is Product D8418, Dimethyl sulfoxide, assayed for DNase, RNase and protease?
Yes. It is a molecular biology grade product, and it is tested for DNase, RNase and protease. For a lot to pass none of those enzymes may be detected.
Why is Product D8418, Dimethyl sulfoxide, considered as an exceptional organic solvent?
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
With what materials is Product D8418, Dimethyl sulfoxide, compatible?
The compatibility of DMSO with various materials is listed below: • Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, PTFE (Teflon) and FEP. • Moderately compatible: polystyrene, ECTFE/ETFE. • Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate. The information is on the product information sheet. Please see the link below: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d8418pis.Par.0001.File.tmp/d8418pis.pdf
DMSO, Product D8418 Dimethyl sulfoxide, is used to freeze and store human and animal cell lines. Is there a procedure for it?
The procedure to freeze cells is in the product information sheet. Please see the link below: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d8418pis.Par.0001.File.tmp/d8418pis.pdf
I received Product D8418, Dimethyl sulfoxide, as a frozen solid. But isn't it supposed to be in the liquid state?
DMSO super cools easily and remelts slowly at room temperature. The product may arrive as a solid instead of a liquid. The solidified product can be reliquified by warming to room temperature without detriment to the product.
Show more questions
Protocols & Articles

Articles

Dimethyl Sulfoxide Molecular Biology Reagent

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Among its uses in organic synthesis is the oxidation of thiols...
Keywords: Amplification, Buffers, Capillary electrophoresis, Centrifugation, Degradations, Electrophoresis, Mass spectrometry, Melting, Molecular biology, Organic synthesis, Oxidations, Phase transitions, Phosphorylations, Polymerase chain reaction, Sequencing, Transfection, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Related Content

Properties of Solvents Table

a. Gant, R., LC/GC 10(7), 515 (1992) 1. At 20 °C 2. At 20 °C +/- 5 °C relative to water at 4 °C 3. At 10mmHg pressure 4. At l=589.32nm
Keywords: Gas chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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