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D8418 Sigma

Dimethyl sulfoxide

for molecular biology

Synonym: DMSO

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Properties

Related Categories β-Glucuronidase Drug Analysis Bundle, Analytical and Industrial Enzymes, Biochemicals, Biochemicals and Reagents, Core Bioreagents,
grade   for molecular biology
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
InChI Key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N
product line   BioReagent
assay   ≥99.9%
form   liquid
autoignition temp.   573 °F
shelf life   Recommended retest period - 2 years
expl. lim.   42 %, 63 °F
impurities   ≤0.001 meq/g Titratable acid
  ≤0.1% water (Karl Fischer)
color   colorless
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
solubility   H2O: miscible (completely)
density   1.10 g/mL(lit.)
absorption   UV absorption/ passes test
suitability   suitable for molecular biology
foreign activity   DNase and RNase, none detected
storage temp.   room temp

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Packaging

1 L in glass bottle

50, 100, 250, 500 mL in glass bottle

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.

Dimethyl sulfoxide has been used-
as an oligonucleotide solvent in array spotting
as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

General description

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
1
RTECS 
PV6210000
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Articles

Dimethyl Sulfoxide Molecular Biology Reagent

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Among its uses in organic synthesis is the oxidation of thiols...
Keywords: Amplification, Buffers, Capillary electrophoresis, Centrifugation, Degradations, Electrophoresis, Mass spectrometry, Melting, Molecular biology, Organic synthesis, Oxidations, Phase transitions, Phosphorylations, Polymerase chain reaction, Sequencing, Transfection, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Peer-Reviewed Papers
15

References

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