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D8437 Sigma

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture



Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Core Bioreagents,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   L-glutamine: yes
HEPES: yes
glucose: yes
phenol red: yes
storage temp.   2-8°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


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Cryostor Cryopreservation Video Protocol

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Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Where can I find the formulation information for Dulbecco’s Modified Eagle’s Medium?
This link will take you to the Formulation page. http://www5.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/Media_Formulations.html
What is Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).
What is the optimal carbon dioxide concentration needed for Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.
Can Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, be used under serum free conditions?
When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).
What is the storage temperature of Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
We recommend storing Product No. D8437 at 2-8°C.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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