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D8764 Sigma

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Synonym: DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
type   Type V
form   essentially salt-free, lyophilized powder
foreign activity   RNase ≤0.1%
storage temp.   −20°C

Description

Packaging

30000, 150000, 300000 units in poly bottle

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM

Application

DNAse II from Sigma has been used in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart.

Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. In vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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