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  • D8815 - Anti-Destrin/ADF (GV-13) antibody produced in rabbit

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D8815 Sigma

Anti-Destrin/ADF (GV-13) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Actin Depolymerizing Factor

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   mouse, human, canine, rat
application(s)   indirect immunofluorescence: suitable using mouse NIH/3T3 fibroblasts
  western blot: suitable using whole extracts of human A-431 epidermoid carcinoma cells, rat PC-12 pheochromocytoma cells, and dog MDCK kidney cells
clone   polyclonal
concentration   ~ 0.5-1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DSTN(11034)
mouse ... Dstn(56431)
rat ... LOC296197(296197)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 153-165 of human destrin/ADF.

General description

Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘Hirano bodies’ in certain brain neurons of dementia patients.

Physical form

Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:1500 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells and for indirect immunofluorescence at a working dilution of 1:100 using mouse NIH/3T3 fibroblasts. It was used in a study to label chick neurons treated with antimycin to examine the colocalization of actin, cofilin, and ADF in pMAP (phosphorylated microtubule-associated protein) inclusions after ATP depletion. It was used to specifically detect ADF in a study.

Features and Benefits

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Biochem/physiol Actions

Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca2+ independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.

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