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D8899 Sigma

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonym: DNA single stranded

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Properties

Related Categories DNA, Mammalian DNA, Molecular Biology, Molecular Biology Reagents
grade   for molecular biology
form   lyophilized powder
mol wt   mol wt ~50 kb
composition   ssDNA, ≥65%
storage temp.   −20°C

Description

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.

Features and Benefits

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Preparation Note

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Quantity

One mg DNA is approx. 25 A260 units.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

Add to Cart

GE27-4575-01 DNA (Calf Thymus), activated, GE Healthcare, 27-4575-01, pack of 1 ea
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