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D8899 Sigma

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonym: DNA single stranded

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Properties

Related Categories DNA, Mammalian DNA, Molecular Biology, Molecular Biology Reagents
grade   for molecular biology
form   lyophilized powder
mol wt   mol wt ~50 kb
composition   ssDNA, ≥65%
storage temp.   −20°C

Description

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.

Features and Benefits

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Preparation Note

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Quantity

One mg DNA is approx. 25 A260 units.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Related Products

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Product #

Description

Add to Cart

GE27-4575-01 DNA (Calf Thymus), activated, GE Healthcare, 27-4575-01, pack of 1 ea
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