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D8900 Sigma

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture

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Description

Application

Suitable for growth of a wide range of cell types in low serum conditions.

Quantity

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability

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powder, with 3151 mg/L dextrose, with 2.5 mM L-glutamine, with 55 mg/L sodium pyruvate, without sodium bicarbonate

With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Safety & Documentation

Safety Information

WGK Germany 
1

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Reconstruction of endometrium from human endometrial side population cell lines. Cervelló I, Mas A, Gil-Sanchis C, et al. PLoS ONE 6(6), e21221, (2011)

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Galectin-3 contributes to luteolysis by binding to Beta 1 integrin in the bovine corpus luteum. Hashiba K, Sano M, Nio-Kobayashi J, et al. Biol. Reprod. 91(1), 2, (2014)

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Expression of adipocyte biomarkers in a primary cell culture models reflects preweaning adipobiology. Chu DT, Malinowska E, Gawronska-Kozak B, et al. J. Biol. Chem. 289(26), 18478-88, (2014)

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Clinical significance of gelsolin-like actin-capping protein expression in oral carcinogenesis: an immunohistochemical study of premalignant and malignant lesions of the oral cavity. Hitomi Nomura et al BMC Cancer 8, 39, (2008)

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Sustained delivery of latanoprost by thermosensitive chitosan-gelatin-based hydrogel for controlling ocular hypertension. Cheng YH, Hung KH, Tsai TH, et al. Acta Biomater. 10(10), 4360-6, (2014)

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Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. Yadav AK, Chaudhari H, Warke H, et al. PLoS ONE 9(10), e108815, (2014)

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Examination of viability and quality of ovarian tissue after cryopreservation using simple laboratory methods in ewe. Merdassi G Reprod. Biol. Endocrinol. 9, 78, (2011)

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Opposing roles of leptin and ghrelin in the equine corpus luteum regulation: an in vitro study. Galvão A, Tramontano A, Rebordão MR, et al. Mediators Inflamm. 2014, 682193, (2014)

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BNP signaling is crucial for embryonic stem cell proliferation. Abdelalim EM PLoS ONE 4(4), e5341, (2009)

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