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D8906 Sigma

Dextran sulfate sodium salt from Leuconostoc spp.

for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

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Description

Application

Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.

Suitability

Tested for suitability in nucleic acid hybridizations

Price and Availability


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Dextran sulfate sodium salt from <I>Leuconostoc</I> spp.

average mol wt >500,000 (dextran starting material), contains 0.5-2.0% phosphate buffer, pH 6-8

Safety & Documentation

Safety Information

WGK Germany 
2
RTECS 
HH9290000

Protocols & Articles

Articles

Dextran and Related Polysaccharides

Historically, dextrans had been long recognized as contaminants in sugar processing and other food production. The formation of dextran in wine was shown by Pasteur to be due to the activity of micro...
Vicki Caligur
BioFiles 2008, 3.10, 17.
Keywords: Adhesion, Applications, Chromatography, Clinical, Cosmetics, Derivatizations, Diffusion, Filtration, Immobilization, Industries, Ion Exchange, Ligands, Microscopy, Molecular biology, Molecular biology techniques, Nuclear magnetic resonance spectroscopy, Nucleic acid hybridization, Pharmaceutical, Precipitation, Purification, Separation, Size-exclusion chromatography, Spectroscopy, Sulfations, Type

Protocols

Dextran Sulfate

Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate gro...
Keywords: Adsorption, Cell proliferation, Esterifications, Fractionation, Immobilization, Molecular biology, Nucleic acid hybridization, Precipitation, Separation

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