|Related Categories||Biochemicals, Biochemicals and Reagents, Blocking Reagents for Hybridization, Carbohydrate adjuvants, Carbohydrates,|
|grade||for molecular biology|
|mol wt||average Mw >500,000 (dextran starting material)|
|contains||0.5-2% phosphate buffer|
|solubility||H2O: soluble100 mg/mL|
Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.
Tested for suitability in nucleic acid hybridizations
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average mol wt 5,000
mol wt 6,500-10,000
average mol wt 9,000-20,000
average mol wt >500,000 (dextran starting material), contains 0.5-2.0% phosphate buffer, pH 6-8
Certificate of Analysis
Certificate of Origin
Historically, dextrans had been long recognized as contaminants in sugar processing and other food production. The formation of dextran in wine was shown by Pasteur to be due to the activity of micro...
BioFiles 2008, 3.10, 17.
Keywords: Adhesion, Chromatography, Clinical, Cosmetics, Derivatizations, Diffusion, Filtration, Immobilization, Industries, Ion Exchange, Ligands, Microscopy, Molecular biology, Molecular biology techniques, Nuclear magnetic resonance spectroscopy, Nucleic acid hybridization, Pharmaceutical, Precipitation, Purification, Separation, Size-exclusion chromatography, Spectroscopy, Sulfations
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Wahl, G.M., et al. Proc. Natl. Acad. Sci. U. S. A. 76, 3683, (1979)
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