Synonym: 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B
|Related Categories||Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Detection, Detection Substrates,|
Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
Customers Also Viewed
Purified for use with peroxidase and peroxidase-coupled reactions.
Suitable for use in glucose determination
for enzymic, spectrophotometric determination, vial of 5 mg
Certificate of Analysis
|Precautionary statements||P201-P308 + P313|
|Personal Protective Equipment||Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges|
|Hazard Codes (Europe)||T|
|Risk Statements (Europe)||45-22|
|Safety Statements (Europe)||53-45|
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
1. OBJECTIVE To standardize a procedure for the determination of the enzymatic assay of Cholesterol Oxidase.
Keywords: Enzyme activity, Extinction coefficient, Isomerizations
Replacing OP SPPUTR01. New document number assigned to conform to current document numbering system.
Keywords: Carcinogens, Extinction coefficient
Derived from procedure SPGLUC01. Includes template updates to current SOP specification and incorporation of notes into the procedure. Refer to CR SOP-DEK ENZ 36.
Keywords: Extinction coefficient
Influence of smoking on interleukin-1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after periodontal treatment. Toker H, Akpınar A, Aydın H, et al. J. Periodont. Res. 47(5), 572-7, (2012)
Euphorbia peroxidase catalyzes thiocyanate oxidation in two different ways, the distal calcium ion playing an essential role. Pintus F, Spanò D, Bellelli A, et al. Biochemistry 49(40), 8739-47, (2010)
Development of new methodologies for on-line determination of the bromate ion in samples of water subjected to ozonation treatment. Almendral MJ, Alonso A, and Fuentes MS J. Environ. Monit. 11(7), 1381-8, (2009)
[New approaches to the measurement of the concentration and peroxidase activity of myeloperoxidase in human blood plasma]. Gorudko IV, Cherkalina OS, Sokolov AV, et al. Bioorg. Khim. 35(5), 629-39, (2009)
Sequential and simultaneous determination of bromate and chlorite (DBPs) by flow techniques: kinetic differentiation. Alonso-Mateos A, Almendral-Parra MJ, and Fuentes-Prieto MS Talanta 76(4), 892-8, (2008)
Hemozymes peroxidase activity of artificial hemoproteins constructed from the Streptomyces lividans xylanase A and iron(III)-carboxy-substituted porphyrins. Ricoux R, Dubuc R, Dupont C, et al. Bioconjug. Chem. 19(4), 899-910, (2008)
Improved analytical sensitivity reveals the occurrence of gender-related variability in diamine oxidase enzyme activity in healthy individuals. García-Martín E, Ayuso P, Martínez C, et al. Clin. Biochem. 40(16-17), 1339-41, (2007)
[Mechanisms of peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate]. Kireĭko AV, Veselova IA, and Shekhovtsova TN Bioorg. Khim. 32(1), 80-6, (2006)
Consumer product in vitro digestion model: Bioaccessibility of contaminants and its application in risk assessment. Brandon EF, Oomen AG, Rompelberg CJ, et al. Regul Toxicol Pharmacol 44(2), 161-71, (2006)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?