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D9154 Sigma

o-Dianisidine dihydrochloride

tablet, 10 mg substrate per tablet

DOWNLOAD MSDS (PDF)

Synonym: 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

  • CAS Number 20325-40-0

  • Empirical Formula (Hill Notation) C14H16N2O2 · 2HCl

  • Molecular Weight 317.21

  •  Beilstein Registry Number 3917996

  •  EC Number 243-737-5

  •  MDL number MFCD00012488

  •  PubChem Substance ID 24894263

  • Popular Documents:  Datasheet (PDF)  |  FTIR (PDF)  

o-Dianisidine dihydrochloride tablet, 10 mg substrate per tablet
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Description

Reconstitution

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

Specificity

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Warning

Probably carcinogenic.

Price and Availability

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