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D9163 Sigma

DL-Dithiothreitol

for electrophoresis, ≥99%

Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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Properties

Related Categories Biochemicals and Reagents, Chemical Synthesis, Core Bioreagents, Denaturation, Disulfide Bridge Cleavage Reagents,
grade   for electrophoresis
InChI Key   VHJLVAABSRFDPM-QWWZWVQMSA-N
assay   ≥99%
mp   40-44 °C
  41-44 °C(lit.)
solubility   H2O: soluble50 mg/mL, clear, colorless to very faintly yellow
cation traces   heavy metals (as Pb): ≤10 ppm
absorption   A0.02M/280 ≤0.04
storage temp.   2-8°C

Description

Analysis Note

Tested for use in reduced SDS-PAGE

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Packaging

1, 5, 25 g in poly bottle

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
EK1610000
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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