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D9170 Sigma

Dimethyl sulfoxide

PCR Reagent

Synonym: DMSO

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Properties

Related Categories Biochemicals and Reagents, Biostabilization Agents, Core Bioreagents, Dimethyl Sulfoxide (DMSO), General Reagents,
grade   PCR Reagent
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
InChI Key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N
assay   ≥99.5%
autoignition temp.   573 °F
shelf life   Recommended retest period - 2 years
expl. lim.   42 %, 63 °F
packaging   vial of 1 mL
color   colorless
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
solubility   H2O: miscible (completely)
density   1.10 g/mL(lit.)
suitability   suitable for PCR
Featured Industry   Agriculture

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Packaging

1 vial in glass bottle

5 vials in glass bottle

Price and Availability


All labs need water
Safety & Documentation
Protocols & Articles

Protocols

Low Contaminant Amplification of DNA Using MTP Taq DNA Polymerase D7442 Protocol

Every precaution should be taken to avoid contamination of reagents with unknown/unwanted DNA. This includes the following:

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