D9170 Sigma

Dimethyl sulfoxide

PCR Reagent

DOWNLOAD MSDS (PDF)

Synonym: DMSO, Methyl sulfoxide

Properties

Related Categories Biochemicals and Reagents, Biostabilization Agents, Core Bioreagents, Dimethyl Sulfoxide (DMSO), General Reagents,
grade   PCR Reagent
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
autoignition temp.   573 °F
expl. lim.   42 %, 63 °F
packaging   vial of 1 mL
refractive index   n20/D 1.479(lit.)
bp   189 °C(lit.)
mp   16-19 °C(lit.)
density   1.10 g/mL(lit.)

Description

Frequently Asked Questions

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Application

Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Packaging

1 vial in glass bottle

5 vials in glass bottle

Price and Availability

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