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D9307 Sigma

JumpStart Taq DNA Polymerase

with MgCl2

Purchase

Properties

Related Categories Core Bioreagents, Hot Start PCR, Life Science Reagents for PCR, Molecular Biology, PCR Enzymes,
form   liquid
feature   hotstart
concentration   2.5 units/μL
color   colorless
  colorless
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Application

• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers

Other Notes

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.

Supplied with 10× reaction buffer containing 15 mM MgCl2

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Features and Benefits

• Reduces non-specific amplification
• Increases PCR specificity and yield
• Reduces set-up time concerns associated with manual or wax Hot Start methods
• Activation time of less than 1 minute

Price and Availability

PCR Selection Guide


PCR Selection GuideNeed help choosing between Taq® and ReadyMix™ products?

Use our interactive PCR selection guide to help you choose the right product for your research needs.




PCR Technical Manual

Genotyping

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Optimized for use with JumpStart Taq DNA Polymerase, D9307.

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Protocols & Articles

Articles

Introduction & Historical Timelines - PCR Technologies Guide

The Polymerase Chain Reaction (PCR) is used in all areas of biological science research, including the clinical, forensic and diagnostic fields and the widespread adoption of the PCR technique has re...
Keywords: Amplification, Clinical, Degradations, Detection methods, Diagnostic, Diseases, Forensic, Gene expression, Genetic, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Separation, Transcription

Protocols

Amplification of DNA Using Jumpstart Taq DNA Polymerase D9307 Protocol

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...

Animal Tissue DNA-Extraction & WGA Amplification Protocol

Animal tissue is a common source of material when performing genetic analysis. The protocol below is a simple method of extracting DNA from the animal sample. Once the DNA has been isolated, it can t...

Antibody-Enzyme Mediated Hot Start PCR Protocol

During PCR assay preparation, nonspecific amplification can occur due to binding of PCR primers to nonspecific templates and from formation of primer dimers which result from using other primer molec...
Keywords: AGE, Amplification, Buffers, Electrophoresis, Enzyme activity, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream...

Buccal DNA Extraction & WGA Amplification Protocol

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample. Once the DNA is is...

Extraction & Amplification of whole blood using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This can hinder the researcher’s ability to perf...

Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via:
Keywords: AGE, Amplification, Electrophoresis, Gel electrophoresis, Gene expression, Nucleic acid annealing, Polymerase chain reaction, Size-exclusion chromatography

Plant DNA extraction & WGA Amplification-protocol

Extracting DNA from plant tissue is a complicated process due to the tough cell wall that surrounds most plant cells. Genomic DNA from plant material can be damaged during the extraction process, res...

Saliva DNA Extraction & WGA Amplification Protocol

Whole Genome Amplification can be performed on DNA extracted in many ways. Sigma-Aldrich offers many products for DNA extraction including the GenElute™ Blood Genomic DNA Kit (NA2010), GenElute Mamma...

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their entire genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our global d...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Agriculture | Plant Breeding Workflow

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

Optimized PCR-based Detection of Mycoplasma [VIDEO]

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest contaminant concerns are mycoplasma contamination. Although mycoplasma do not usually kill cont...
Keywords: Detection methods, Metabolism, Polymerase chain reaction

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Disruption of Abi1/Hssh3bp1 expression induces prostatic intraepithelial neoplasia in the conditional Abi1/Hssh3bp1 KO mice. Xiong X, Chorzalska A, Dubielecka PM, et al. Oncogenesis 1, e26, (2012)

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Genomic DNA functions as a universal external standard in quantitative real-time PCR. Yun, J.J., et al. Nucleic Acids Res. 34, e85, (2006)

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Two Hsp70 family members expressed in atherosclerotic lesions. Han, Z., et al. Proc. Natl. Acad. Sci. U. S. A. 100, 1256, (2003)

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Simplified hot start PCR. Birch, D.E., et al. Nature 381, 445, (1996)

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Homologous recombination DNA repair genes play a critical role in reprogramming to a pluripotent state. González F, Georgieva D, Vanoli F, et al. Cell Rep. 3(3), 651-60, (2013)

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Generation of human induced pluripotent stem cells bearing an anti-HIV transgene by a lentiviral vector carrying an internal murine leukemia virus promoter. Kamata M, Liu S, Liang M, et al. Hum. Gene Ther. 21(11), 1555-67, (2010)

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A sensitive procedure to detect alternatively spliced mRNA in pooled-tissue samples. Leparc GG and Mitra RD Nucleic Acids Res. 35(21), e146, (2007)

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Helicobacter Pylori's plasticity zones are novel transposable elements. Dangeruta Kersulyte et al PLoS ONE 4, e6859, (2009)

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Comparison of eight methods for the extraction of Bacillus atrophaeus spore DNA from eleven common interferents and a common swab. Helen L. Rose et al PLoS ONE 6, e22668, (2011)

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Restoration of adenosine deaminase-deficient human thymocyte development in vitro by inhibition of deoxynucleoside kinases. Joachims ML J. Immunol. 181(11), 8153-61, (2008)

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