• USA Home
  • D9380 - DNA Polymerase I from Escherichia coli lysogenic for NM 964

EMAIL THIS PAGE TO A FRIEND
D9380 Sigma

DNA Polymerase I from Escherichia coli lysogenic for NM 964

buffered aqueous glycerol solution

Synonym: Kornberg Polymerase

Purchase

Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   buffered aqueous glycerol solution
mol wt   mol wt 109 kDa
concentration   5,000-15,000 units/mL
foreign activity   Endonuclease, none detected
shipped in   wet ice
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... polA(948356)

Description

Components

DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol.

Application

Suitable for:
• Highly specific DNA probes by nick translation
• In vitro synthesis of complementary cDNA strand
• In vitro synthesis of DNA
• Produce blunt ends from 5′ and 3′ overhangs

General description

DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.

Unit Definition

One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37 °C.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

P6911 Protease from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)
LIG1 DNA Ligation Kit
R0884 T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution
D8276 DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution
D2886 DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution
P4978 Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution
T4427 Terminal Transferase from calf thymus, buffered aqueous glycerol solution
P4390 Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution
E1131 Exonuclease III from Escherichia coli BE25 /psGR3, buffered aqueous glycerol solution
G5663 Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
D7291 Deoxyribonuclease I RNase-free solution from bovine pancreas
T7428 Topoisomerase I from wheat germ, buffered aqueous glycerol solution
R6513 Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
R4642 Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?