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D9542 Sigma

DAPI

for nucleic acid staining

Synonym: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, 4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI dihydrochloride

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Properties

Related Categories Apoptosis Detection, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Fluorescent,
Quality Level   PREMIUM
grade   for molecular biology
InChI Key   FPNZBYLXNYPRLR-UHFFFAOYSA-N
assay   ≥98% (HPLC and TLC)
form   powder
solubility   H2O: soluble20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
  PBS: insoluble
absorption   ε1mM/263 nm, H2O 30
fluorescence   λex 340 nm; λem 488 nm (nur DAPI)
  λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability   suitable for fluorescence
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caution

Protect from light.

Packaging

1, 5, 10, 50 mg in glass bottle

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Suitable for
• DNA staining in agarose gels
• analysis of changes in DNA during apoptosis
• detection of mycoplasma
• photofootprinting of DNA
• immunofluorescent staining of cells

DAPI has been used:-
• in rapid monitoring of microbial contamination
• in chromosomal banding technique
• in detection of apoptotic cells
• in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
• to stain mature pollen grains(0.5 mg/ml)

General description

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

Price and Availability


Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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