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D9655 Sigma

pBR322 Hae III Digest

for DNA electrophoresis

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Properties

Related Categories DNA Digest Markers, Markers and Ladders, Molecular Biology, Nucleic Acid Electrophoresis
form   liquid
usage   1000 uses (0.5 mg size)
  200 uses (0.1 mg size)
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

General description

Sigma’s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

Contains 22 fragments, 8–587 bp

For optimal resolution, the recommended agarose gel concentration is 2.5.

Preparation Note

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

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