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D9785 Sigma

DMEM/Nutrient Mixture F-12 Ham

With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

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Properties

Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   glucose: 3.15 g/L
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
L-glutamine: no
NaHCO3: no
phenol red: no
storage temp.   2-8°C

Description

General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.

Quantity

Formulated to contain 14.8 grams of powder per liter of medium.

Reconstitution

Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

Protocols & Applications

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Peer-Reviewed Papers
15

References

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F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
L6914 L-Leucine, PharmaGrade, Ajinomoto, EP, JP, USP, Manufactured under appropriate GMP controls for pharma or biopharmaceutical production, suitable for cell culture
M8439 L-Methionine, PharmaGrade, Ajinomoto, EP, JP, USP, manufactured under appropriate GMP controls for Pharma or Biopharmaceutical production, suitable for cell culture
L5501 L-Lysine, ≥98% (TLC)
M2643 Magnesium sulfate, BioReagent, suitable for cell culture, suitable for insect cell culture
C5670 Calcium chloride, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
S5761 Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

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