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D9785 Sigma

DMEM/Nutrient Mixture F-12 Ham

With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham



Related Categories Cell Biology, Cell Culture, Classic Media and Salts, Classical Media, Culture,
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   glucose: 3.15 g/L
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
L-glutamine: no
NaHCO3: no
phenol red: no
storage temp.   2-8°C


General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.


Formulated to contain 14.8 grams of powder per liter of medium.


Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

Protocols & Applications

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability

All labs need water
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Peer-Reviewed Papers


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