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D9793 Sigma

Lambda DNA BstE II Digest

buffered aqueous solution

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Properties

grade   for molecular biology
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

Contains 14 fragments, 117–8,454 bp

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA

Application

BstE II digested bacteria phage Lambda DNA is suitable for size determination of double-stranded DNA using DNA electrophoresis. The recommended agarose gel concentration is 0.7% for this marker. A sample of the marker should be diluted with gel loading buffer to an appropriate loading concentration. Typically 500 ng per well (50 ng/μl, 10 μl load) is sufficient to be seen using ethidium bromide staining.

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