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D9893 Sigma

pBR322 Plasmid DNA from E. coli RRI

lyophilized powder

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Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for Cloning, Research Essentials
grade   for molecular biology
form   lyophilized powder
mol wt   mol wt 2.9 MDa
  mol wt 4363 bp
storage temp.   2-8°C

Description

Components

DNA is provided as a powder, lyophilized from a solution of 1 mM Tris-HCl (pH 7.5) with 1mM NaCl and 1mM EDTA.

Application

Suitable for use in:
• Cloning
• Selection and expression of recombinant molecules
• Construction of shuttle vectors
• Construction of vectors for nucleotide sequencing
• Studies of elements involved in gene expression
• As plasmid DNA standards
• As a model system for studies on prokaryotic plasmid replication

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.

General description

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.

The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).

Other Notes

Accession number: J01749

Accession number: J01749

Specificity

Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.

Biochem/physiol Actions

This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.

Price and Availability

Protocols & Articles
Peer-Reviewed Papers
15

References

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D4904 pBR322 Plasmid DNA from E. coli RRI, buffered aqueous solution

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