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D9905 Sigma

Denhardt’s Solution

for Northern and Southern blotting, lyophilized powder

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Properties

Related Categories Biochemicals, Blocking Reagents for Hybridization, Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting,
grade   for molecular biology
form   lyophilized powder
foreign activity   DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.   2-8°C

Description

Components

Each vial of Denhardt’s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

General description

Denhardt’s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Packaging

5 mL in glass bottle

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

Application

Suitable as a blocking Buffer for Southern and Northern blot membranes.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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