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DMN10 Sigma

GenElute Direct mRNA Miniprep Kits

sufficient for 10 purifications

Synonym: Gen Elute, GenElute Direct mRNA Miniprep Kit, GenElute mRNA Kit

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Description

Application

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Other Notes

Use for isolating mRNA directly from mammalian cells or tissues.

For additional information, please see www.sigma-aldrich.com/mrna.

Features and Benefits

• mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
• Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
• Oligo(dT) polystyrene beads require fewer wash steps

General description

The GenElute Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


D-Tube Dialyzers

Microcon Centrifugal Filters

All labs need water

Standard component

Description

Product #

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Elution solution 1.5 mL SDS    
Filtration columns with tubes 10 ea SDS    
Lysis solution 20 mL SDS    
5 M NaCl 1.5 mL SDS    
Oligo(dT)-polystyrene beads .3 mL SDS    
Proteinase K 5 mg SDS    
40% Glycerol solution .6 mL SDS    
Spin columns with tubes 10 ea SDS    
Collection tube 10 ea SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

Protocols

Reverse Transcription Using ReadyScript cDNA Synthesis RDRT Protocol

Preparation Place components on ice. Mix, and then briefly centrifuge to collect contents at the bottom of the tube

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