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DNAQF Sigma

DNA Quantitation Kit, Fluorescence Assay

Quantitation of DNA using bisBenzimide

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Properties

Related Categories Cell Biology, DNA & RNA Purification, General Stem Cell Biology, Molecular Biology, Nucleic Acid Quantitation,
usage   sufficient for 750 assays (2 ml each)
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Features and Benefits

• Specific for the quantitation of nanogram amounts of DNA
• Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins

Application

All the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins.

Price and Availability


Microcon Centrifugal Filters

All labs need water

Standard component

Description

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Detailed technical bulletin 1 ea SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
I see that one of the componets of Product DNAQF, DNA Quantitation Kit, Fluorescence Assay, is a DNA standard. How is the DNA standard prepared? Can it be purchased separately?  
The DNA standard (Product No. D4810) used in the kit is a sheared calf thymus DNA. It is the only one tested with the kit and it is only available in the kit. We do not determine the molecular weight.
How can I ensure accurate initial quantification of the DNA standard by spectrophotometric measurement at A260 when using Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?
The DNA standard (Product No. D4810) should be incubated at 50 °C for 15-30 minutes before determining the concentration spectrophotometrically. This will ensure complete dissolution of the genomic DNA.
What quality of genomic DNA is required for use in Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?
Genomic DNA purified by phenol chloroform extraction is sufficiently clean for use with this kit.
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Protocols & Articles

Articles

A Novel 3D Perfusion Bioreactor

Qing Liu, Ph.D., is an expert in biomaterials, tissue engineering, and in vitro tissue model for drug screening. Carlos E. Caicedo-Carvajal, Ph.D., has worked in several areas of Biomedical Engineeri...
Carlos E. Caicedo-Carvajal, Ph.D.; Qing Liu, Ph.D.
Biowire Spring 2012, 25–28
Keywords: Adhesion, Biomaterials, Cancer, Cell culture, Cell proliferation, Deposition, Gene expression, Nutrition, Size-exclusion chromatography

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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