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DUO92103 Sigma

Duolink® In Situ Red Starter Kit Mouse/Goat

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Properties

Related Categories Duolink PLA Technology, Duolink Starter Kits, Molecular Biology, Protein Interaction, Proteomics More...
fluorescence   λex 594 nm; λem 624 nm (Texas Red®; Zeiss Filter set 31)
suitability   suitable for fluorescence

Description

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), red detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink® In Situ Red Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Features and Benefits

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Legal Information

Duolink is a registered trademark of Sigma-Aldrich Co. LLC

PLA is a registered trademark of Sigma-Aldrich Co. LLC

Texas Red is a registered trademark of Life Technologies

Price and Availability


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Kit component also available separately

Description

Product #

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Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L) SDS DUO92006
Duolink® In Situ PLA® Probe Anti-Mouse PLUS, Affinity purified Donkey anti-Mouse IgG (H+L) SDS DUO92001
Duolink® In Situ Detection Reagents Red SDS DUO92008
Duolink® In Situ Wash Buffers, Fluorescence SDS DUO82049
Duolink® In Situ Mounting Medium with DAPI SDS DUO82040
Protocols & Articles

Articles

Duolink® FAQs

From our library of Articles, Sigma-Aldrich presents Duolink® FAQs
Keywords: Addition reactions, Bacterial conjugations, Dehydration reaction, Filtration, Immunohistochemistry, PAGE, Purification, Titrations

Duolink® PLA Applications

With Duolink® PLA, you can detect, quantify, and visualize protein-protein interactions, post-translational modifications, and low expression protein detection with an easy-to-execute immunodetection...
Keywords: Acylations, Biological processes, Buffers, DNA replication, Electrophoresis, Gel electrophoresis, Gene expression, Glycosylations, High performance liquid chromatography, Immunohistochemistry, Mass spectrometry, Metabolism, Phosphorylations, Post translational modifications, Sulfations, Transcription, Transduction, Ubiquitinations

Duolink® PLA Technology for Neuroscience Applications

Neurons are dynamic entities, sensing and responding to their immediate environment. For this reason, understanding protein interactions and signaling mechanisms is a focus of neuroscience research a...
Keywords: Genetic, Neuroscience, Peptide synthesis

Duolink® References

Duolink proximity ligation assay (PLA) has been used in countless studies in a variety of fields. Browse the following tables to view highlights from the many applications of this technology.
Keywords: Apoptosis, Cancer, Cell biology, Coagulation, DNA replication, Degradations, Epigenetics, Gene expression, Growth factors, Immunology, Methylations, Neuroscience, Oxidations, PAGE, Peptide synthesis, Phosphorylations, Polymorphisms, Post translational modifications, Transcription

Protocols

Duolink® In Situ Short Instructions - Fluorescence

Note: Use open droplet reactions without a cover slip and perform all incubations in a humidity chamber. Use volumes corresponding to your reaction area, see the Reaction Volume Guide at sigma.com/du...
Keywords: Amplification, Confocal microscopy, Peptide synthesis, Phase transitions

Duolink® PLA Fluorescence Protocol

This protocol describes the use of Duolink® PLA reagents for the immunofluorescent detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions...
Keywords: Amplification, Buffers, Confocal microscopy, Gene expression, Microscopy, Peptide synthesis, Titrations

Duolink® PLA Product Selection Guide

Getting started with Duolink® PLA is simple. The protocols are very similar to that of IF, IHC, and flow cytometry, however Duolink® PLA offers distinct advantages over these and other traditional me...
Keywords: Amplification, Bacterial conjugations, Buffers, Flow cytometry, Fluorescent microscopy, Immunohistochemistry, Microscopy, Peptide synthesis

Duolink® PLA Troubleshooting Guide

The Duolink® PLA technology for protein detection offers a simple and straightforward protocol that has a set of defined steps. This section will delineate important reminders that will ensure your D...
Keywords: Addition reactions, Amplification, Bacterial conjugations, Buffers, Dehydration reaction, Filtration, Flow cytometry, Immunofluorescence, Immunohistochemistry, Peptide synthesis, Purification, Titrations

How to Optimize the Duolink® Proximity Ligation Assay

To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol.
Keywords: Bacterial conjugations, Buffers, Detergents, Flow cytometry, Fluorescent microscopy, Gene expression, Growth factors, Immunofluorescence, Immunohistochemistry, In Situ hybridization, Microscopy, Nucleic acid hybridization, Phosphorylations, Post translational modifications, Sample preparations

Related Content

Duolink® PLA Resource Center

Understanding protein function in the cell can create solutions to improve human health. For that reason we created the Duolink® PLA Resource Center, to provide insightful educational resources so yo...
Keywords: Neuroscience

Neurodegenerative Disease Analysis with Duolink® [VIDEO]

As the population ages, neurodegenerative diseases are becoming increasingly prevalent. These diseases bear with them increased health-care costs, caretaker burden, and an urgent research need. Resea...
Keywords: Diseases, Neurodegenerative Diseases

Search Primary Antibodies for use with Duolink® PLA

The use of high quality, primary antibodies is critical to success for running a Duolink® Proximity Ligation Assay. We offer over 300 PLA-validated antibodies and antibody pair kits, in addition to t...
Keywords: Immunohistochemistry, PAGE, Peptide synthesis

Peer-Reviewed Papers
15

References

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