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E0127 Sigma

Elastase from porcine pancreas

Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein

Synonym: Elastase from hog pancreas, Pancreatopeptidase E

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Description

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides, p-dinitrophenyl diethylphosphate and high salt concentrations. Elastase is extensively used in tissue and cell dissociation procedures. It is effective in the isolation of Type II lung cells.

Packaging

10, 20 mg in poly bottle

5 mg in glass bottle

Package size based on protein content

100 mg in glass bottle

Preparation Note

2x crystallized and chromatographically purified

Quality

Contains trypsin activity

Unit Definition

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Physical form

Contains sodium carbonate.

Application

Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.

The enzyme from Sigma has been used in the proteolytic cleavage of UMP synthase (Uridine-5′-monophosphate synthase). It has also been used along with collagenase to disperse tissue into single cells from the thoracic media, abdominal aortic media and intima of rabbit.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange
Keywords: Chromatography, High performance liquid chromatography

Protocols

Elastase (EC 3.4.21.36) continuous spectrophotometric rate assay

The continuous spectrophotometric rate determination (A410, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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M9771 N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, elastase substrate
S4760 N-Succinyl-Ala-Ala-Ala-p-nitroanilide, elastase substrate
S8511 N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide, protease substrate

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