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  • E0143 - Monoclonal Anti-Episialin (EMA) antibody produced in mouse

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E0143 Sigma

Monoclonal Anti-Episialin (EMA) antibody produced in mouse

clone GP1.4, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formalin-fixed, paraffin-embedded sections of human breast carcinoma
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  microarray: suitable
clone   GP1.4, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MUC1(4582)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human milk fat globule membranes.

General description

Episialin (mucin-1) is an epithelial transmembrane oncoprotein that occurs in glandular tissues. This oncoprotein mediates ErbB1 receptor transport and subsequently regulates cell survival. Episialin also modulates p53-responsive genes under conditions of genotoxic stress and also co-activates the transcription of Wnt target genes. Overexpression of Episialin has been associated with most human cancers
Monoclonal Anti-Episialin (EMA) antibody is specific for the repetitive protein epitope on the episialin long extracellular domain. This epitope is relatively insensitive to glycosylation. The antibody stains 2 bands (ranging between 265-400 kD) by immunoblotting. The product also reacts with highly glycosylated episialin in epithelial cell tumors and large cell anaplastic lymphomas. In immunohistological assays, enzymatic pre-treatment of formalin-fixed, paraffin-embedded sections may enhance the staining intensity.

Application

Monoclonal Anti-Episialin (EMA) antibody is suitable for use in immunohistochemistry (1:200 using formalin-fixed, paraffin-embedded sections of human breast carcinoma), immunoprecipitation and microarray.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Peer-Reviewed Papers
15

References

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