Synonym: Elastase from hog pancreas, Pancreatopeptidase E
|Related Categories||3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Signaling Enzymes,|
|foreign activity||trypsin ≤50 BAEE units/mg protein|
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.1
Package size based on protein content
A further purification of Type III, E 0127, by affinity chromatography to reduce trypsin activity
One unit will hydrolyze 1.0 μmole of N-succinyl-
Contains sodium carbonate.
Elastase from porcine pancreas has been used in a study to assess the molecular bases for human leucocyte elastase inhibition. Elastase from porcine pancreas has also been used in a study to investigate the molecular cloning and expression of serum calcium-decreasing factor (caldecrin).
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lyophilized powder, ≥50 units/mg protein (Bradford)
lyophilized powder, BioReagent, suitable for cell culture
Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein
Type II-A, lyophilized powder, Protein ~70 %, ≥1 units/mg protein (biuret)
powder, white, ≥15 U/mg
Certificate of Analysis
Certificate of Origin
2. SCOPE This assay procedure applies to all products that have specification for the enzymatic activity of Elastase using SucALA3 as a substrate at Sigma-Aldrich Saint Louis.
Keywords: Enzyme activity, Extinction coefficient
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
1. Elastase D.M. Shotton Meth. Enzymol. 19, 113-140, (1970)
Influence of secretory leukocyte protease inhibitor-based peptides on elastase activity and their incorporation in hyaluronic acid hydrogels for chronic wound therapy. Barros SC, Martins JA, Marcos JC, et al. Biopolymers 98(6), 576-90, (2012)
A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine. Chicooree N, Griffiths JR, Connolly Y, et al. Rapid Commun. Mass Spectrom. 27(1), 127-34, (2013)
Commentary on 'Performance of a modified rabbit model of abdominal aortic aneurysm induced by topical application of porcine elastase: 5-month follow-up study'. Cockerill GW Eur. J. Vasc. Endovasc. Surg. 45(2), 153, (2013)
Performance of a modified rabbit model of abdominal aortic aneurysm induced by topical application of porcine elastase: 5-month follow-up study. Bi Y, Zhong H, Xu K, et al. Eur. J. Vasc. Endovasc. Surg. 45(2), 145-52, (2013)
Genetic and pharmacologic disruption of interleukin-1β signaling inhibits experimental aortic aneurysm formation. Johnston WF, Salmon M, Su G, et al. Arterioscler. Thromb. Vasc. Biol. 33(2), 294-304, (2013)
Peptide inhibitor of CXCL4-CCL5 heterodimer formation, MKEY, inhibits experimental aortic aneurysm initiation and progression. Iida Y, Xu B, Xuan H, et al. Arterioscler. Thromb. Vasc. Biol. 33(4), 718-26, (2013)
The utility of lung epithelium specific biomarkers in cardiac surgery: a comparison of biomarker profiles in on- and off-pump coronary bypass surgery. Engels GE, Gu YJ, van Oeveren W, et al. J. Cardiothorac. Surg. 8(1), 4, (2013)
Potent elastase inhibitors from cyanobacteria: structural basis and mechanisms mediating cytoprotective and anti-inflammatory effects in bronchial epithelial cells. Salvador LA, Taori K, Biggs JS, et al. J. Med. Chem. 56(3), 1276-90, (2013)
[Purification and physicochemical properties of Bacillus thuringiensis IMB B-7324 peptidase with elastolytic and fibrinolytic activity]. Matseliukh OV, Nidialkova NA, and Varbanets' LD Ukr. Biokhim. Zh. 84(6), 25-36, (2012)
[Peculiarities of the circulating phagocytes functional activity in patients with different forms of drug-resistant pulmonary tuberculosis]. Titarenko OT, D'iakova ME, Ésmedliaeva DS, et al. Biomed. Khim. 58(4), 467-74, (2012)
Long-range electrostatic complementarity governs substrate recognition by human chymotrypsin C, a key regulator of digestive enzyme activation. Batra J, Szabó A, Caulfield TR, et al. J. Biol. Chem. 288(14), 9848-59, (2013)
[The blood biochemical indicators as possible markers of destruction of kerato-hyaline tissue of nails in patients with onycholysis]. Urazovskaia EV and Mikashinovich ZI Klin. Lab. Diagn. (12), 8-10, (2012)
Hemodynamics and axial strain additively increase matrix remodeling and MMP-9, but not MMP-2, expression in arteries engineered by directed remodeling. Nichol JW, Khan AR, Birbach M, et al. Tissue Eng. Part A 15(6), 1281-90, (2009)
Prevention of hepatocarcinogenesis and increased susceptibility to acetaminophen-induced liver failure in transaldolase-deficient mice by N-acetylcysteine. Hanczko R J. Clin. Invest. 119(6), 1546-57, (2009)
Measurement of local strains in intervertebral disc anulus fibrosus tissue under dynamic shear: contributions of matrix fiber orientation and elastin content. Michalek AJ, Buckley MR, Bonassar LJ, et al. J. Biomech. 42(14), 2279-85, (2009)
Human leukocyte and porcine pancreatic elastase: X-ray crystal structures, mechanism, substrate specificity, and mechanism-based inhibitors. Bode W, Meyer E, and Powers JC Biochemistry 28(5), 1951-63, (1989)
Active site mapping of the serine proteases human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II. Bovine chymotrypsin A alpha, and Staphylococcus aureus protease V-8 using tripeptide thiobenzyl ester substrates. Harper, J.W., et al. Biochemistry 23, 2995, (1984)
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