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  • E0521 - Anti-Estrogen Receptor (18-32) antibody produced in rabbit

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E0521 Sigma

Anti-Estrogen Receptor (18-32) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Hormones, Antibodies to Steroid Hormones and Related Products,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:3000 using MCF7 cultured cell line extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 67 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ESR1(2099)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 18-32 of the human estrogen receptor.

General description

Estrogen is a sex hormone that is a key regulator of growth, differentiation and function of reproductive tissues, skeletal and cardiovascular systems. The effect of estrogen is mediated by two intracellular receptors, ERα and ERβ. Although the two receptors interact with similar ligands, have identical DNA-binding domains and have redundant roles in estrogen signaling, they show distinct expression pattern. On binding to the receptor, the estrogen-ER complex enters nucleus and regulates the transcription of specific genes. The biological effects of estrogen and ER is by upstream activation of the pathway by growth factors. Some pathways that activate the estrogen-ER pathway are those mediated by AP-1, IGF-1, EGF, MAPK and PI3K/Akt . Anti-Estrogen Receptor (18-32) antibody is specific for 18-32 amino acids of human estrogen receptor (67 kDa). The product is an IgG fraction prepared from pooled antisera.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide.

Application

Anti-Estrogen Receptor (18-32) antibody may be used for immunoblotting using extracts of MCF7 cells at a working dilution of 1:3000. It may also be used for dot blot.

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