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E0526 Sigma

Endonuclease III from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, buffered aqueous glycerol solution, ≥10,000 units/mg protein

Synonym: Nth protein, Endo III

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
recombinant   expressed in E. coli overproducing strain
assay   ≥90% (SDS-PAGE)
form   buffered aqueous glycerol solution
mol wt   mol wt ≥23 kDa (211 amino acids)
shipped in   wet ice
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... nth(947122)

Description

Preparation Note

Purified in an iron bound form that confers the protein a light green color.

Unit Definition

One unit will cleave 50% of 0.5 pmol double strand oligonucleotide substrate containing 5,6-dihydrothymine in 15 min. at 30 °C.

Physical form

Solution in 20 mM Tris, pH 7.4, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% glycerol.

Biochem/physiol Actions

Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme. It possesses DNA glycosylase activity with broad substrate specificity for mutated pyrimidine derivatives especially thymine glycol (Tg) but also for 5-hydroxycytosine and 5,6-dihydrothymine. In addition, it has an apurinic/apyrimidinic (AP) lyase activity which cleaves the DNA backbone by β elimination. Endo III protects E. coli cells from radiation damage caused by x-ray and UV irradiation and its activity overlaps the activity of endonuclease VIII. When overexpressed, it can also protect E.coli cells from alkylation defects caused by methylmethanesulphonate.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
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