• USA Home
  • E0648 - pcDNA 3.1 (−)/Myc−His C expression vector

EMAIL THIS PAGE TO A FRIEND
E0648 Sigma

pcDNA 3.1 (−)/Myc−His C expression vector

Purchase

Properties

grade   for molecular biology
form   buffered aqueous solution
mol wt   mol wt 5500 bp
storage temp.   −20°C

Description

Components

pcDNA 3.1 (-)/Mys-His C Expression Vector is supplied lyophilized in 10 mM Tris-HCl and 1 mM EDTA (pH 7.5).

Features and Benefits

• CMV promoter/enhancer permits efficient, high-level expression
• MCS facilitates easy insertion of your gene
• Myc epitope allows recombinant protein detection
• His-tag allows recombinant protein purification (on metal chelate resins)
• Confers ampicillin resistance for selection of transformed bacteria
• Confers neomycin resistance for selection of tranfected mammalian cells
• SV40 promoter allows high level expression of inserted gene and episomal replication in cells expressing the large T antigen

A 5.5-kb vector for protein expression in mammalian cells. The vector incorporates both the myc epitope and polyHistidine in a C-terminal fusion protein tag. The A, B, and C versions differ in only a single base pair, allowing for translation in all possible reading frames.
All three contain the strong cytomegalovirus (CMV) enhancer-promoter sequence for high-level expression, the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability, and the SV40 replication origin.

General description

pcDNA3.1 (-)/myc-his C is an expression vector that contains the strong CMV enhancer-promoter for high level expression of recombinant proteins in mammalian cells. The pcDNA3.1(-)/myc-his C expression vector contains a multiple cloning site (MCS) for easy cloning. pcDNA3.1(-) /myc-his C expression vector has a multiple cloning site in an opposite orientation from the pcDNA3.1(+). The vector is supplied in one of three reading frames to facilitate in frame cloning with a C-terminal tag containing a polyhistidine metal-binding region and the myc (c-myc) epitope.

The MCS has the following unique restriction sites: Apa I, Asp718 I, BamH I, BstX I, EcoR I, EcoR V, Hind III, KpN I, Nhe I, Not I, Pme I, Xba I, and Xho I. In addition, the SnaBI site in the CMV promoter is unique and the BstXI site in the MCS is redundant.

Specificity

The MCS, containing Apa I, Asp718 I, BamH I, BstX I, EcoR I, EcoR V, Hind III, KpN I, Nhe I, Not I, Pme I, Xba I, Xho I restriction sites, is in the reverse orientation. In addition, there is a unique SnaB I site in the CMV promoter.

Application

pcDNA 3.1 (-)/Mys-His C Expression Vector is suitable for expression of recombinant proteins in mammalian cells.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?