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E0887 Sigma

Esterase from rabbit liver

lyophilized powder, ≥75 units/mg protein

Synonym: Carboxyl esterase, Carboxylic-ester hydrolase

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Description

Packaging

500, 1000 units in poly bottle

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Application

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).

The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.

Biochem/physiol Actions

Esterase is a gylcoprotein having a molecular mass of approximately 60 kDa and is mainly present in the endoplasmic reticulum of hepatocytes. The enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Esterase

To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Keywords: Addition reactions, Titrations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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Related Products

substrate

Product #

Description

Add to Cart

N0758 Naphthol AS-D chloroacetate, esterase substrate
N8000 1-Naphthyl butyrate, esterase substrate
B4377 5-Bromo-4-chloro-3-indolyl acetate, esterase substrate
I3500 Indoxyl acetate, ≥95% (GC)
N8130 4-Nitrophenyl acetate, esterase substrate

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