Benzonase® Nuclease
≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
DOWNLOAD MSDS (PDF)Synonym: Endonuclease from Serratia marcescens
-
CAS Number 9025-65-4
-
EC Number 231-791-2
-
MDL number MFCD00131010
| 
Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Lysis and Protein Extraction Reagents, |
| recombinant | expressed in E. coli |
| assay | ≥90% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| concentration | ≥250 units/μL |
| foreign activity | protease, essentially free |
| storage temp. | −20°C |
Description
Application
Used for the removal of nucleic acid from protein samples.
Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no photolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorymetric experiments.
Biochem/physiol Actions
Digests native or heat-denatured DNA and RNA.
Legal Information
Benzonase® Nuclease is supplied by Merck KGaA and its affiliates.
Benzonase is a registered trademark of Merck KGaA
Unit Definition
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).
Physical form
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
Price and Availability
Customers Also Viewed
≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
For bacterial cell lysis, standard strength
For bacterial lysis
Related Papers
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
