EMAIL THIS PAGE TO A FRIEND

E1014 Sigma

Benzonase® Nuclease

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonym: Endonuclease from Serratia marcescens

Purchase

Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Lysis and Protein Extraction Reagents,
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   buffered aqueous glycerol solution
concentration   ≥250 units/μL
foreign activity   protease, essentially free
storage temp.   −20°C

Description

Application

Used for the removal of nucleic acid from protein samples.

Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorimetric experiments.

The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures. It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Benzonase® is a genetically engineered endonuclease from Serratia marcescens.1,2 The protein is a dimer of 30 kDa subunits with two essential disulfide bonds.3,4,5,6 This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is found to be 8.0-9.2. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids.

Legal Information

Benzonase® Nuclease is supplied by Merck KGaA and its affiliates.

Benzonase is a registered trademark of Merck KGaA

Unit Definition

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).

Physical form

Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.

Price and Availability

Timestrip - Time & Temperature Indicators


Timestrip
Discover how Timestrip® indicators will help you monitor expiry dates or excessive temperatures.




TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
  • . Superior Band Resolution
  • . Strong & Tear Resistant
  • . Higher Capacity Wells


Additional Products to Explore

Deoxyribonuclease I from bovine pancreas

Type IV, lyophilized powder, ≥2,000 Kunitz units/mg protein

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

OptiPrep<SUP>™</SUP> Density Gradient Medium

used for cell and subcellular organelle isolation

Phosphodiesterase I from <I>Crotalus adamanteus</I> venom

vial of ≥0.40 units, Purified

for use with mammalian cell and tissue extracts, DMSO solution

Customers Also Viewed

Benzonase<SUP>®</SUP> Nuclease, ultrapure

≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade

For bacterial cell lysis, standard strength

OptiPrep<SUP>™</SUP> Density Gradient Medium

used for cell and subcellular organelle isolation

Phosphodiesterase I from <I>Crotalus adamanteus</I> venom

vial of ≥0.40 units, Purified

Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 
1

Protocols & Articles

Articles

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, Methods, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Spectra, Transfection, Western blot

Related Content

Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.

Find enzymes / proteins, substrates, activators, and inhibitors. Resources for metabolic pathways, kinases, proteolytic enzymes and inhibitors, carbohydrate analysis as well as new cell signaling, an...
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. ISOLATION AND PROPERTIES OF AN EXOCELLULAR NUCLEASE OF SERRATIA MARCESCENS. Eaves GN and Jeffries CD J. Bacteriol. 85(2), 273-8, (1963)

Loading...


2. An extracellular nuclease from Serratia marcescens. II. Specificity of the enzyme. Nestle M and Roberts WK J. Biol. Chem. 244(19), 5219-25, (1969)

Loading...


3. 2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA. Miller MD, Tanner J, Alpaugh M, et al. Nat. Struct. Biol. 1(7), 461-8, (1994)

Loading...


4. A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Friedhoff P, Gimadutdinow O, Rüter T, et al. Protein Expr. Purif. 5(1), 37-43, (1994)

Loading...


5. Disulfide bonds are required for Serratia marcescens nuclease activity. Ball TK, Suh Y, and Benedik MJ Nucleic Acids Res. 20(19), 4971-4, (1992)

Loading...


6. The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli. Ball TK, Saurugger PN, and Benedik MJ Gene 57(2-3), 183-92, (1987)

Loading...


Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Molloy, M.P., et al. Electrophoresis 19, 837, (1998)

Loading...


Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Herbert, B.R. Electrophoresis 19, 845-851, (1998)

Loading...


Benzonase--possibility of practical application. Olszewski, M. and P. Filipkowski. Postepy Biochem. 55, 21-4, (2009)

Loading...


Nuclease biosynthesis and growth of Serratia marcescens in the presence of 2-(p-aminobenzenesulfonamide)-thiazole. Starshinova , N. and M. Filimonova. Mikrobiologiia 74, 365-9, (2005)

Loading...


HLA-targeted flow cytometric sorting of blood cells allows separation of pure and viable microchimeric cell populations. Drabbels JJ, van de Keur C, Kemps BM, et al. Blood 118(19), e149-55, (2011)

Loading...


Plk1 phosphorylation of TRF1 is essential for its binding to telomeres. Weber, G., et al. J. Biol. Chem. 283, 25503-25513, (2008)

Loading...


Voltammetric trace analysis of DNA and RNA. Reher, S., et al. Fresenius J. Anal. Chem. 368, 720-6, (2000)

Loading...


Labeling of multiple HIV-1 proteins with the biarsenical-tetracysteine system. Pereira CF, Ellenberg PC, Jones KL, et al. PLoS ONE 6(2), e17016, (2011)

Loading...


[Expression, purification and characterization of non-specific Serratia nuclease in Escherichia coli]. Chen P, Yang H, Li H, et al. Sheng Wu Gong Cheng Xue Bao 27(8), 1247-57, (2011)

Loading...


[Transgenic tobacco (Nicotiana tabacum SR1) plants expressing the gene coding for Serratia marcescens nuclease]. Trifonova EA, Komarova ML, Syrnik OA, et al. Genetika 38(2), 274-7, (2002)

Loading...


[A comparative structure-function analysis and molecular mechanism of action of endonucleases from Serratia marcescens and Physarum polycephalum]. Shliapnikov SV, Lunin VV, Blagova EV, et al. Bioorg. Khim. 28(1), 23-31, (2002)

Loading...


Long-term and age-dependent restoration of visual function in a mouse model of CNGB3-associated achromatopsia following gene therapy. Livia S. Carvalho et al Hum. Mol. Genet. 20, 3161-75, (2011)

Loading...


Fluorescence-quenching-based enzyme-activity assay by using photon upconversion. Rantanen, T., et al. Angew. Chem. Int. Ed. Engl. 47, 3811-3813, (2008)

Loading...


Progress in understanding the biology of the human mutagen LINE-1. Babushok DV and Kazazian HH Hum. Mutat. 28(6), 527-39, (2007)

Loading...


Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding proteins. Thomas MP, Whangbo J, McCrossan G, et al. J. Immunol. 192(11), 5390-7, (2014)

Loading...


Mice deficient in the serine/threonine protein kinase VRK1 are infertile due to a progressive loss of spermatogonia. Wiebe MS Biol. Reprod. 82(1), 182-93, (2010)

Loading...


Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of chlorite dismutase: a detoxifying enzyme producing molecular oxygen. de Geus DC, Thomassen EA, van der Feltz CL, et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64(Pt 8), 730-2, (2008)

Loading...


Downstream processing of lentiviral vectors: releasing bottlenecks. Bandeira V, Peixoto C, Rodrigues AF, et al. Hum. Gene Ther. Methods 23(4), 255-63, (2012)

Loading...


Efficient removal of DNA from proteomic samples prior to two-dimensional map analysis. Antonioli P, Bachi A, Fasoli E, et al. J. Chromatogr. A 1216(17), 3606-12, (2009)

Loading...


A proteogenomic analysis of Shigella flexneri using 2D LC-MALDI TOF/TOF. Zhao L, Liu L, Leng W, et al. BMC Genomics 12, 528, (2011)

Loading...


GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons. Ho VM, Dallalzadeh LO, Karathanasis N, et al. Mol. Cell. Neurosci. 61, 1-12, (2014)

Loading...


Polymerase chain reaction, nuclease digestion, and mass spectrometry based assay for the trinucleotide repeat status of the fragile X mental retardation 1 gene. Dodds ED, Tassone F, Hagerman PJ, et al. Anal. Chem. 81(13), 5533-40, (2009)

Loading...


A simple and effective method to generate lentiviral vectors for ex vivo gene delivery to mature human peripheral blood lymphocytes. Yang S, Karne NK, Goff SL, et al. Hum. Gene Ther. Methods 23(2), 73-83, (2012)

Loading...


Separation and identification of trinucleotide-melphalan adducts from enzymatically digested DNA using HPLC-ESI-MS. Mohamed D and Linscheid M Anal. Bioanal. Chem 392(5), 805-17, (2008)

Loading...


A BEN-domain-containing protein associates with heterochromatin and represses transcription. Sathyan KM, Shen Z, Tripathi V, et al. J. Cell Sci. 124(Pt 18), 3149-63, (2011)

Loading...


GenoMass--a computer software for automated identification of oligonucleotide DNA adducts from LC-MS analysis of DNA digests. Liao Q, Shen C, and Vouros P J. Mass Spectrom. 44(4), 549-60, (2009)

Loading...


Systematic functional comparative analysis of four single-stranded DNA-binding proteins and their affection on viral RNA metabolism. Shi H, Zhang Y, Zhang G, et al. PLoS ONE 8(1), e55076, (2013)

Loading...


Separation and characterization of oxaliplatin dinucleotides from DNA using HPLC-ESI ion trap mass spectrometry. Mowaka S and Linscheid M Anal. Bioanal. Chem 392(5), 819-30, (2008)

Loading...


Epitope Characterization And Variable Region Sequence Of F1-40, A High-affinity Monoclonal Antibody To Botulinum Neurotoxin Type A (Hall Strain). Scotcher, M.C., et al. PLoS ONE 4, e4924, (2009)

Loading...


Clearance and characterization of residual HSV DNA in recombinant adeno-associated virus produced by an HSV complementation system. Ye GJ, Scotti MM, Liu J, et al. Gene Ther. 18(2), 135-44, (2011)

Loading...


A conditional suicide system for Saccharomyces cerevisiae relying on the intracellular production of the Serratia marcescens nuclease. Balan A and Schenberg AC Yeast 22(3), 203-12, (2005)

Loading...


Solvent participation in Serratia marcescens endonuclease complexes. Chen C, Beck BW, Krause K, et al. Proteins 62(4), 982-95, (2006)

Loading...


Production and comprehensive quality control of recombinant human Interleukin-1beta: a case study for a process development strategy. Block H, Kubicek J, Labahn J, et al. Protein Expr. Purif. 57(2), 244-54, (2008)

Loading...


Quantitative determination of the infectivity of the proviral DNA of a retrovirus in vitro: Evaluation of methods for DNA inactivation. Sheng-Fowler L, Lewis AM, and Peden K Biologicals 37(4), 259-69, (2009)

Loading...


Action of hexaamminecobalt on the activity of Serratia marcescens nuclease. Filimonova M, Gubskaya V, Nuretdinov I, et al. Biometals 16(3), 447-53, (2003)

Loading...


Evaluation of the Serratia marcescens nuclease (NucA) as a transgenic cell ablation system in porcine. Caballero I and Piedrahita JA Anim. Biotechnol. 20(4), 177-85, (2009)

Loading...


Post-ischemic administration of peptide with apurinic/apyrimidinic endonuclease activity inhibits induction of cell death after focal cerebral ischemia/reperfusion in mice. Kim HW, Cho KJ, Lee BI, et al. Neurosci. Lett. 460(2), 166-9, (2009)

Loading...


Mung bean nuclease I. 3. Purification procedure and (3') omega monophosphatase activity. Mikulski AJ and Laskowski M J. Biol. Chem. 245(19), 5026-31, (1970)

Loading...


Gradation of specificity with regard to sugar among nucleases. Wechter WJ, Mikulski AJ, and Laskowski M Biochem. Biophys. Res. Commun. 30(3), 318-22, (1968)

Loading...


Manufacture of measles viruses. Langfield KK, Walker HJ, Gregory LC, et al. Methods Mol. Biol. 737, 345-66, (2011)

Loading...


DNA digestion to deoxyribonucleoside: a simplified one-step procedure. Quinlivan EP and Gregory JF Anal. Biochem. 373(2), 383-5, (2008)

Loading...


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?