Synonym: Endonuclease from Serratia marcescens
|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Lysis and Protein Extraction Reagents,|
|recombinant||expressed in E. coli|
|form||buffered aqueous glycerol solution|
|foreign activity||protease, essentially free|
Used for the removal of nucleic acid from protein samples.
Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no photolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorymetric experiments.
Digests native or heat-denatured DNA and RNA.
Benzonase® Nuclease is supplied by Merck KGaA and its affiliates.
Benzonase is a registered trademark of Merck KGaA
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
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≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
For bacterial cell lysis, standard strength
For bacterial lysis
lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein
used for cell and subcellular organelle isolation
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Transfection, Western blot
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