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E1157 Sigma

Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-EGFR, Anti-ERBB, Anti-HER1, Anti-PIG61

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Epidermal Growth Factors (EGF) and NRG Family,
species reactivity   human
application(s)   capture ELISA: 0.4 μg/mL
  flow cytometry: 25 μg/mL
  immunoprecipitation: 1 μg/mL
  western blot: 1-2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... EGFR(1956)
biological source   goat
conjugate   unconjugated

Description

Immunogen

purified, NSO cell-derived recombinant human epidermal growth factor receptor extracellular domain.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Application

Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat was used for immunoblotting to analyze stable expression of anti-EGFR shRNA in MCF10A breast epithelial cell line.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Features and Benefits

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Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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