E1404 Sigma

Embryonic Stem Cells: Methods and Protocols

  •  ISBN-10 0-89603-881-5

  •  ISBN-13 978-0-89603-881-3

Properties

publication info   K. Turksen, ed., Humana Press, 2002, 512 pp., hard cover

Description

General description

This book describes productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. These detailed protocols range from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, to the generation of antibodies against very rare antigens, and to the identification of and characterization of protein and protein interactions. This will prove an invaluable resource for those actively using, or planning to use ES cells, to study fate choices and specific lineages.

Table of Contents

1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells
2 The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos
3 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
4 Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation
5 Effects of Altered Gene Expression on ES Cell Differentiation
6 Hypoxic Gene Regulation in Differentiating ES Cells
7 Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells
8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development
9 Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System
10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
11 Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells
12 Embryonic Stem Cell Differentiation and the Vascular Lineage
13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation
14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications
15 Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
16 Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells
17 Lineage Selection for Generation and Amplification of Neural Precursor Cells
18 Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson′s Disease
19 Epidermal Lineage
20 ES Cell Differentiation Into the Hair Follicle Lineage In Vitro
21 Embryonic Stem Cells as a Model for Studying Melanocyte Development
22 Using Progenitor Cells and Gene Chips to Define Genetic Pathways
23 ES Cell-Mediated Conditional Transgenesis
24 Switching on Lineage Tracers Using Site-Specific Recombination
25 From ES Cells to Mice: The Gene Trap Approach
26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells
27 Phage-Displayed Antibodies to Detect Cell Markers
28 Gene Transfer Using Targeted Filamentous Bacteriophage
29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors
30 Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays
31 Expression Profiling Using Quantitative Hybridization on Macroarrays
32 Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells
33 Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
34 Direct Selection of cDNAs by Phage Display
35 Screening for Protein–Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells
Index

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