Ethidium bromide solution
BioReagent, for molecular biology, 10 mg/mL in H2O
DOWNLOAD MSDS (PDF)Synonym:
3,8-
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CAS Number 1239-45-8
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Empirical Formula (Hill Notation) C21H20BrN3
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Molecular Weight 394.31
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Beilstein Registry Number 3642536
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MDL number MFCD00011724
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PubChem Substance ID 24894418
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Popular Documents: Specification Sheet (PDF) | FTIR (PDF)
Properties
| Related Categories | Agarose Blends for Electrophoresis, Biochemicals and Reagents, Core Bioreagents, Ethidium Bromide, Fluorescent Probes, Labels, Particles and Stains, |
| grade | for molecular biology |
| product line | BioReagent |
| concentration | 10 mg/mL in H2O |
| suitability | suitable for gel electrophoresis |
Description
Application
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.1,2,3 It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.5
Biochem/physiol Actions
Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Packaging
10 mL in glass bottle
Reconstitution
For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.
Price and Availability
Nucleic Acid Electrophoresis Reagents
Find all the necessary reagents to perfomyour Nucleic Acid Electrophoresis research
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