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  • E1523 - Anti-ERK5 (big-MAPK, BMK1) antibody produced in rabbit

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E1523 Sigma

Anti-ERK5 (big-MAPK, BMK1) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, EO-ER,
species reactivity   human, mouse
application(s)   microarray: suitable
  western blot: 1:8,000 using NIH 3T3 mouse fibroblasts cell extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 97 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK7(5598)
mouse ... Mapk7(23939)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 789-802 of human ERK5.

General description

Extracellular signal-regulated kinase 5 (ERK5), also referred to as big-MAP kinase 1 or BMK1, is a protein that belongs to Mitogen-activated protein kinases family and is expressed mainly in skeletal muscle, kidney, heart and placenta. It has a critical role in different cellular processes like differentiation, proliferation and survival. Anti-ERK5 (big-MAPK, BMK1) antibody can be used to study the differential tissue expression and intracellular localization of the MAP Kinase isoform in normal and neoplastic tissues. Rabbit anti-ERK5 (big-MAPK, BMK1) antibody reacts specifically with ERK5 (97 kD) from mouse fibroblast cell extract.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-ERK5 (big-MAPK, BMK1) antibody can be used in immunoblotting to detect and localize ERK5 from whole cell extract of NIH 3T3 mouse fibroblasts. It can also be used in microarray.

Features and Benefits

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