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E1532 Sigma

Monoclonal Anti-E2F6 antibody produced in mouse

~1.0 mg/mL, clone TFE61, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-E2F transcription factor 6, Anti-E2F-6

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors, CB - E999,
species reactivity   human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  western blot: 2-4 μg/mL using HeLa nuclear cell extract
clone   TFE61, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~35 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... E2F6(1876)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human E2F6 protein

General description

E2F6 transcription factor belongs to the third class of E2Fs. The E2F transcription factors are a family of genes that play critical roles in regulation of cellular proliferation and differentiation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal Anti-E2F6 antibody produced in mouse is suitable for immunocytochemistry and indirect ELISA. It is also suitable for western blotting at a working concentration of 2-4μg/mL using HeLa nuclear cell extract.

Features and Benefits

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Biochem/physiol Actions

The third class of E2Fs lacks both the retinoblastoma protein binding and transcriptional activation domain, and is an active repressor of transcription. The activity of E2F6 is mediated through its recruitment of polycomb transcriptional repressor complexes in a histone methylation independent manner. E2F6 associates with E2F target genes that are activated at G1/S phase. Its repression activity during the S-phase distinguishes G1/S and G2/M transcription during cell cycle. It may be involved in various cancerous processes.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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WH0001876M1 Monoclonal Anti-E2F6 antibody produced in mouse, clone 2B6-G9, purified immunoglobulin, buffered aqueous solution

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