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E166 Sigma

Monoclonal Anti-Endothelin-1 antibody produced in mouse

clone TR.ET.48.5, purified antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurotransmission, Antibodies to Neuropeptides, EJ-EN,
species reactivity   sheep, human, rat
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250
  radioimmunoassay: 1:25,000
  western blot: suitable
clone   TR.ET.48.5, monoclonal
antibody form   purified antibody
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EDN1(1906)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

endothelin-1 (ET-1) conjugated to KLH

General description

Endothelin consists of a family of potent vasoconstrictor peptides, which include four structurally related isoforms, ET-1, ET-2, ET-3 and vasointestinal contractor (VIC, β -ET)

Physical form

Solution in phosphate buffered saline, containing 0.05% sodium azide

Specificity

The antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides.

Application

Monoclonal Anti-Endothelin-1 antibody produced in mouse is suitable for immunoblotting and immunocytochemistry.It is also suitable for radioimmunoassay (RIA) at a working dilution of 1:25,000 and for immunohistochemistry at 1:250 working dilution using formalin-fixed, paraffin-embedded tissue sections.

Features and Benefits

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Biochem/physiol Actions

ET-1, a 21 amino acid peptide, is produced by vascular endothelial cells. It is a potent vasoconstrictor and plays an important role in the homeostasis of the circulatory system. It is also important in pathogenesis of cardiovascular diseases. It is also involved in contraction of airway and intestinal smooth muscle. It releases vasodilator prostaglandins and nitric oxide (NO). It also plays a role in mitogenic effects on vascular smooth muscle cells and fibroblasts, stimulation of atrial natriuretic peptide secretion from atrial cardiocytes and inhibition of renin release. ET-1 is expressed in the spinal cord and dorsal root ganglia, where it may serve as a neurotransmitter/neuromodulator.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

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Peer-Reviewed Papers
15

References

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