E2129 Sigma

Evans Blue

Dye content ≥75 %

Synonym: Direct Blue 53



Related Categories Cell Biology, Core Bioreagents, E-F, Hematology and Histology, Research Essentials,
form   powder
composition   Dye content, ≥75%
Gene Information   human ... PTPN1(5770), PTPRF(5792)


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


10, 50, 100 g in glass bottle


Evans blue has been used to check cell viability. It has been used to study BBB permeability.

Biochem/physiol Actions

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.

Price and Availability

Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How should Product E2129, Evans Blue, be dissolved?
This product is soluble in water, alcohol, acids and alkalies.
What is the solution stability of Product E2129, Evans Blue?
This product is rather stable in aqueous solution and may be autoclaved at 15 psi for 30 min. However, dye made up in physiological saline should not be autoclaved.
What is the difference between Evans Blue Products E2129 and 206334?
Both products are Evans Blue. Product No. E2129 has a dye content of 80% and Product No. 206334 has a dye content of approximately 85%.
What is the shelf life of Product E2129, Evans Blue?
This product has a retest date of 4 years.  This information can be found on the Certificate of Analysis.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Can Evans Blue, Product E2129, be used as a counterstain for FITC?
This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988).
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Protocols & Articles
Peer-Reviewed Papers


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