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E2275 Sigma

pFLAG-CMV−6a,b,c Expression Vectors

set of pFLAG-CMV-6a, -6b, and -6c vectors for cytoplasmic expression of N-terminal FLAG

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Properties

Related Categories Cloning and Expression, Core Bioreagents, Expression Vectors, For Transient Expression, Life Science Reagents for Transfection,
grade   for molecular biology
form   buffered aqueous solution
conjugate   FLAG® tagged
shipped in   dry ice
storage temp.   −20°C

Description

Components

• p-FLAG-CMV-6a Expression Vector (20μg)
• p-FLAG-CMV-6b Expression Vector (20 μg)
• p-FLAG-CMV-6c Expression Vector (20 μg)
• pFLAG-CMV-6a-BAP control plasmid (20 μg)

Application

p-FLAG-CMV-6a, p-FLAG-CMV-6b, and p-FLAG-CMV-6c Expression Vectors are suitable for cytoplasmic expression of N-terminal FLAG® tagged proteins.

General description

p-FLAG-CMV-6a, p-FLAG-CMV-6b, and p-FLAG-CMV-6c Expression Vectors used for the expression of N-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-6 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of N-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.

Vector Maps and Sequences

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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