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E2511 Sigma

ExtrAvidin®

essentially salt-free, lyophilized powder

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Properties

Related Categories Antibodies, Avidin / Biotin Reagents, Biotin/Avidin, ExtrAvidin Reagents, Molecular Biology,
form   essentially salt-free, lyophilized powder
application(s)   dot blot: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  radioimmunoassay: suitable
shipped in   wet ice
storage temp.   2-8°C

Description

Other Notes

A modified form of affinity purified egg white avidin.

Specificity

Binding Activity: at least 10 μg biotin per mg

Application

ExtrAvidin® may be used in conjunction with biotin in techniques such as ELISA, RIA, immunohistochemistry, protein blotting, and with DNA probes. It was used to capture biotinylated RNA in a study to understand translation initiation pathway using immobilised mRNA analogues. It was used to bind to biotinylated surface during preparation of substrates for protein assembly.

Biochem/physiol Actions

ExtrAvidin® is prepared from egg white avidin. It is a tetrameric protein containing four high affinity binding sites for biotin. It combines the high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. It binds biotin with the high affinity of egg white avidin, however, it does not exhibit the unwanted non-specific binding reported for egg white avidin at physiological pH, such as the staining of mast cells.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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