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  • E2520 - Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse

E2520 Sigma

Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse

clone EGF-10, purified immunoglobulin, buffered aqueous solution


Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   dot blot: 0.1 μg/mL using recombinant human EGF
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue
  indirect ELISA: suitable
  microarray: suitable
  neutralization: suitable
  radioimmunoassay: suitable
  western blot: suitable
clone   EGF-10, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 6 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EGF(1950)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology



recombinant human EGF

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4


Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.


Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.

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Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

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