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E2635 Sigma

Anti-Epidermal Growth Factor antibody produced in rabbit

whole antiserum, aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   mouse
application(s)   enzyme immunoassay: suitable
  immunohistochemistry (frozen sections): suitable
  radioimmunoassay: 1:1,000
clone   polyclonal
antibody form   whole antiserum
form   aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Egf(13645)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

highly purified EGF from mouse submaxillary glands.

General description

Epidermal growth factor (EGF) is a growth factor that acts as mitogen for various types of cells of epithelial origin. EGF binds to EGF receptor and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. Some of the downstream pathways activated by EGF/EGF R are PLCγ, PI3K, STAT3, PKC and MAPK cascades. There are numerous effector molecule activated by EGF that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Anti-Epidermal Growth Factor antibody is whole rabbit antiserum.

Physical form

Solution containing 15 mM sodium azide

Specificity

This antiserum may be used to monitor EGF levels in biological fluids, organs and cell culture media.

Application

Anti-Epidermal Growth Factor antibody may be used to monitor EGF levels in organs, biological fluids, and cell culture media by means of radioimmunoassay (RIA), enzyme immunoassay (EIA) and immunohistological techniques. The antiserum may be used as a tool to determine purity when isolating EGF. Antibody dilution of 1:1000 to 1:5000 may be used for RIA. The antibody was used for immunohistochemistry of mouse salivary gland and liver sections at a working dilution of 1:100 and 1:1500, respectively. A dilution of 1:250 of this antibody was used to inject into female mice to study the process of embryo growth.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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E1157 Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat, affinity isolated antibody, lyophilized powder

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